FAQ: When does public transportation operate in Israel?
Public bus transportation in the Tel Aviv area begins to operate every day from 5am, and the last route on Sunday-Thursday usually runs at midnight. On Fridays, bus services finish their last routes of the day in the mid-afternoon (a few hours before sundown) and resume on Saturday night, shortly after sundown (the end of the Sabbath).
On the day leading up to a holiday that begins at night and on the day of the holiday itself (such as Passover, Rosh Hashanah, Yom Kippur, Sukkot, and Shavuot), buses follow a schedule similar to the Friday-Saturday timetable.
Note: To travel on public buses, you need to have Rav-Kav ticket. You can purchase it from the driver on the bus for one-way trip and after that you need to put money on it at ticket booths in the bus stations or in Superpharm stores.